Acta Biologica

Previously: Zeszyty Naukowe Uniwersytetu Szczecińskiego. Acta Biologica


ISSN: 2450-8330     eISSN: 2353-3013    OAI    DOI: 10.18276/ab.2021.28-10
CC BY-SA   Open Access   DOAJ

Issue archive / No. 28
Phytochemical analysis, antioxidant and anticancer activity of Aerva javanica growing in district Karak, Khyber Pakhtunkhwa Pakistan

Authors: Sajida Afzal ORCID
Kohat University of Science and technology Kohat, Department of Zoology

Siraj Khan ORCID
Department of Botany, Abdulwali Khan University

Majid Iqbal ORCID
Institute of Geographic Science and Natural Resources Research UCAS

Anam Akhtar ORCID
Department of Plant Sciences, Quaid- i- Azam University
Keywords: phytochemistry antioxidant potential anticancer potential GC-MS analysis Aerva javanica
Data publikacji całości:2021
Page range:17 (91-107)
Cited-by (Crossref) ?:

Abstract

The purpose of this study was to evaluate the photochemical, antioxidants and anticancer activity of the medicinal plant Aerva javanica. This plant belongs to the Amaranthaceae family. Locally it is called “bui”. It is a shrub with a long tap root that grows all over India in the wild. The plant extracts were prepared using ethanol, methanol and distilled water as solvents. The antioxidant activity was determined using DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical scavenging activity and IC50 was determined. The total flavonoids compounds found in Aerva javanica ethanolic extract were (0.90 ±0.16) while the total phenolic contents found in ethanolic extract were (0.78 ±0.16), followed by the methanolic and aqueous extract. The antioxidant results of methanolic extract of Aerva javanica showed 0.78 ±0.18 percent inhibition and SCV 49.10% at concentration of 1.5 mg/ml, ethanolic extract showed 0.54 ±0.12 percent inhibition with 64.28% SCV. Phytochemical analysis of best result oriented Aerva javanica extract was done with Gas Chromatography-Mass Spectrometry (GC-MS) technique. The results revealed the presence of different compounds predominantly Acetone (1.18%), Ethyl Acetate (38.95%), (20.77%), n-Propyl acetate (4.09%), Isobutyl acetate (2.71%), (3.84%), isoquinoline,1-[(3,4-diethoxyphenyl)methyl]-6,7-diethoxy- (3.36%), Cyclohexanone (1.43%), 1,1-Diisobutoxy-isobutane (2.02%), n-Hexadecanoic acid (5.61%), Phytol (3.57%), 9-Octadecenoic acid, 1,2,3-propanetriyl ester (10.72%), Octadecanoic acid (1.78%), Bis(2-ethylhexyl) phthalate (3.48%), Squalene (1.40%), 2,2-Dimethyl-3-(3,7,16,20-tetramethyl (1.12%) and 1,6,10,14,18,22-Tetracosahexaen-3-ol (1.195%). The study concludes that extensive research is required to detect more novel compounds in order to develop effective management approaches that significantly reduce the impact of the pathogens on human health as well as on environment.
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Bibliography

1.Bajpai, S.K., Jadaun, M., Tiwari, S. (2016). Synthesis, characterization and antimicrobial applications of zinc oxide nanoparticles loaded gum acacia/poly (SA) hydrogels. Carbohydrate polymers, 153, 60–65.
2.Demirgan, R., Karagöz, A., Pekmez, M., Önay-Uçar, E., Artun, F.T., Gürer, Ç., Mat, A. (2016). In vitro anticancer activity and cytotoxicity of some papaver alkaloids on cancer and normal cell lines. African Journal of Traditional, Complementary and Alternative Medicines, 13 (3), 22–26.
3.Dey, A., Nandy, S., Mukherjee, A., Modak, B.K. (2021). Sustainable utilization of medicinal plants and conservation strategies practiced by the aboriginals of Purulia district, India: a case study on therapeutics used against some tropical otorhinolaryngologic and ophthalmic disorders. Environment, Development and Sustainability, 23 (4), 5576–5613.
4.El-Newary, S.A. (2016). The hypolipidemic effect of Portulaca oleracea L. stem on hyperlipidemic Wister Albino rats. Annals of Agricultural Sciences, 61 (1), 111–124.
5.Ezhilan, B.P., Neelamegam, R. (2012). GC-MS analysis of phytocomponents in the ethanol extract of Polygonum chinense L. Pharmacognosy Research, 4 (1), 11–14.
6.Firenzuoli, F., Gori, L. (2007). Herbal medicine today: clinical and research issues. Evidence-based complementary and alternative medicine: eCAM, 4 (Suppl 1), 37–40. DOI: 10.1093/ecam/nem096.
7.Fridlender, M., Kapulnik, Y., Koltai, H. (2015). Plant derived substances with anti-cancer activity: from folklore to practice. Frontiers in plant science, 6, 799–807.
8.Gopalakrishnan, S., Kalaiarasi, T. (2013). Determination of biologically active constituents of the fruits of Cucumis sativus Linn. using GC-MS analysis. International Journal of Biological & Pharmaceutical Research, 4 (7), 523–527.
9.Hojjati, M., Barzegar, H. (2017). Chemical composition and biological activities of lemon (Citrus limon) leaf essential oil. Nutrition and Food Sciences Research, 4 (4), 15–24.
10.Ismail, M., Ibrar, M., Iqbal, Z., Hussain, J., Hussain, H., Ahmed, M., Ejaz, A., Choudhary, M.I. (2009). Chemical constituents and antioxidant activity of Geranium wallichianum. Records of Natural Products, 3 (4), 193–197.
11.Janaki, A., Kaleena, P.K., Elumalai, D., Velu, K., Babu, M., Ravi, S., Arulvasu, C. (2018). Qualitative and quantitative phytochemical analysis: In vitro studies of antioxidant and anticancer activity of Bauhinia tomentosa (L) leaf extracts. J. Pharmacogn. Phytochem, 7 (5), 2403–2410.
12.Karade, D., Vijayasarathi, D., Kadoo, N., Vyas, R., Ingle, P.K., Karthikeyan, M. (2020). Design of Novel Drug-like Molecules Using Informatics Rich Secondary Metabolites Analysis of Indian Medicinal and Aromatic Plants. Combinatorial Chemistry & High Throughput Screening, 23 (10), 1113–1131.
13.Kilcar, A.Y., Yildiz, O., Dogan, T., Sulu, E., Takan, G., Muftuler, F.Z. (2020). The effect of bitter melon (Momordica charantia) extract on the uptake of 99mtc labeled paclitaxel: In vitro monitoring in breast cancer cells. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal ChemistryAnti-Cancer Agents), 20 (12), 1497–1503.
14.Maqsood, M., Qureshi, R., Ikram, M., Ahmad, M.S., Jabeen, B., Asi, M.R., Lilge, L. (2018). In vitro anticancer activities of Withania coagulans against HeLa, MCF-7, RD, RG2, and INS-1 cancer cells and phytochemical analysis. Integrative medicine research, 7 (2), 184–191.
15.Maqsood, S., Adiamo, O., Ahmad, M., Mudgil, P. (2020). Bioactive compounds from date fruit and seed as potential nutraceutical and functional food ingredients. Food Chemistry, 3 (08), 125–522.
16.Mukherjee, P.K., Nema, N.K., Maity, N., Sarkar, B.K. (2013). Phytochemical and therapeutic potential of cucumber. Fitoterapia, 84, 227–236.
17.Okafor, I.A., Ezejindu, D.N. (2014). Phytochemical studies on Portulaca oleracea (purslane) plant. GJBAHS, 3 (1), 132–136.
18.Pandian, R.S., Noora, A.T. (2019). GC-MS Analysis of Phytochemical Compounds Present in the Leaves of Citrus medica. L. Research Journal of Pharmacy and Technology, 12 (4), 1823–1826.
19.Rehman, J., Khan, I.U., Farid, S., Kamal, S., Aslam, N. (2013). Phytochemical screening and evaluation of in-vitro antioxidant potential of Monotheca buxifolia. E3 Journal of Biotechnology and Pharmaceutical Research, 4 (4), 54–60.
20.Riaz, U., Iqbal, S., Sohail, M.I., Samreen, T., Ashraf, M., Akmal, F., Akhter, R.M. (2021). A comprehensive review on emerging importance and economical potential of medicinal and aromatic plants (MAPs) in current scenario. Pakistan Journal of Agricultural Research, 34 (2), 381–392.
21.Russo, D., Miglionico, R., Carmosino, M., Bisaccia, F., Andrade, P.B., Valentão, P., Milella, L., Armentano, M.F. (2018). A comparative study on phytochemical profiles and biological activities of Sclerocarya birrea (A. Rich.) Hochst leaf and bark extracts. International journal of molecular sciences, 19 (1), 186.
22.Saha, A., Basak, B.B. (2020). Scope of value addition and utilization of residual biomass from medicinal and aromatic plants. Industrial Crops and Products, 145, DOI: 10.1016/j.indcrop.2019.111979.
23.Saidu, A.N., Oibiokpa, F.I., Olukotun, I.O. (2014). Phytochemical screening and hypoglycemic effect of methanolic fruit pulp extract of Cucumis sativus in alloxan induced diabetic rats. Journal of Medicinal Plants Research, 8 (39), 1173–1178.
24.Sani, I., Bello, F., Abdul-Kadir, D. (2014). Phytochemical Screening and Antibacterial Activity of Allium sativum, Calotropis procera, Acacia nilotica, and Mitracarpus scaber Mixed Hexane Extracts. World J. Pharmaceut. Res, 3 (10), 142–149.
25.Sarwar, M.R., Iftikhar, S., Saqib, A. (2018). Availability of anticancer medicines in public and private sectors, and their affordability by low, middle and high-income class patients in Pakistan. BMC cancer, 18 (1), 1–11.
26.Sarwar, M.D., Xia, Y.X., Liang, Z.M., Tsang, S.W., Zhang, H.J. (2020). Mechanistic pathways and molecular targets of plant-derived anticancer ent-kaurane diterpenes. Biomolecules, 10 (1), 144.
27.Shaikh, A.M., Shrivastava, B., Apte, K.G., Navale, S.D. (2016). Medicinal plants as potential source of anticancer agents: a review. Journal of Pharmacognosy and Phytochemistry, 5 (2), 291–295.
28.Stefanelli, P., Barbini, D.A., Girolimetti, S. (2021). Pesticides and their metabolites in human urine: development of multi-analyte method by LC-MS/MS and GC-MS/MS. Journal of Environmental Science and Health, Part B, 56 (5), 431–438.
29.Veeru, P., Kishor, M.P., Meenakshi, M. (2009). Screening of medicinal plant extracts for antioxidant activity. Journal of Medicinal Plants Research, 3 (8), 608–612.
30.Wang, A., Li, R., Ren, L., Gao, X., Zhang, Y., Ma, Z., Luo, Y. (2018). A comparative metabolomics study of flavonoids in sweet potato with different flesh colors (Ipomoea batatas (L.) Lam). Food chemistry, 260, 124-–134.
31.Williams, W,B., Cuvelier, M.E., Berset, C.L.W.T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28 (1), 25–30.
32.Wolfe, K., Wu, X., Liu, R.H. (2003). Antioxidant activity of apple peels. Journal of agricultural and food chemistry, 51 (3), 609–614.
33.Youdim, K.A., Shukitt-Hale, B., Joseph, J.A. (2004). Flavonoids and the brain: interactions at the blood– brain barrier and their physiological effects on the central nervous system. Free Radical Biology and Medicine, 37 (11), 1683–1693.
34.Zhu, Q., Nakagawa, T., Kishikawa, A., Ohnuki, K., Shimizu, K. (2015). In vitro bioactivities and phytochemical profile of various parts of the strawberry (Fragaria× ananassa var. Amaou). Journal of Functional Foods, 13, 38–49.